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InVivoMAb Anti-Human Melanotransferrin/CD228/MFI2 Antibody (Iv0104)
Overview
Catalog No. HY094010
Brand abinScience
Tested applications FCM
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS pH 7.4
Purity >95% as determined by SDS-PAGE.
Isotype IgG1
Applications ELISA, FCM, Neutralization
Target CD228, Melanotransferrin, Melanoma-associated antigen p97, MAP97, MFI2, MELTF
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level Please contact with the lab for this information.
Accession P08582
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Clone IDIv0104
Note For research use only. Not suitable for clinical or therapeutic use.
Isotype Control Human IgG1 (LALAPG) Recombinant Isotype Control Antibody (HyHEL-10) (HP688307)
Images


Bioactivity
Detects CD228/MELTF in indirect ELISAs.

Flow Cytometry
Flow-cytometry using anti-human CD228 antibody.A431 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD228 antibody monoclonal antibody (Catalog # HY094010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE
SDS-PAGE for InVivoMAb Anti-Human CD228/MELTF (Iv0104).

Flow CytoMetry
Flow-cytometry using PE anti-human CD228 antibody. A431 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD228 monoclonal antibody (Catalog: HY094010, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

Flow CytoMetry
Flow-cytometry using FITC anti-human CD228 antibody. A431 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD228 monoclonal antibody (Catalog: HY094010, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

Flow CytoMetry
Flow-cytometry using APC anti-human CD228 antibody. A431 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD228 monoclonal antibody (Catalog: HY094010, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.