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InVivoMAb Anti-Human ARG1/Arginase-1 Antibody (Iv0120)
Overview
Catalog No. HY339010
Brand abinScience
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS pH 7.4
Purity >95% as determined by SDS-PAGE.
Isotype IgG2a, kappa
Applications ELISA, FCM, Neutralization
Target Liver-type arginase, ARG1, Type I arginase, Arginase-1
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level Please contact with the lab for this information.
Accession P05089
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Clone IDIv0120
Note For research use only. Not suitable for clinical or therapeutic use.
Isotype Control Mouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023)
Images


Bioactivity		 Detects Human ARG1/Arginase-1 in indirect ELISAs.

Flow Cytometry		 Flow-cytometry using anti-human Arginase 1 antibody.M2 macrophages derived from THP-1 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human Arginase 1 antibody monoclonal antibody (Catalog # HY339010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow Cytometry		 Flow-cytometry using anti-human Arginase 1 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human Arginase 1 antibody monoclonal antibody (Catalog # HY339010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE		 SDS-PAGE for InVivoMAb Anti-Human ARG1/Arginase-1 Antibody (Iv0120).

Flow CytoMetry		 Flow-cytometry using PE anti-human Arginase 1 antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human Arginase 1 monoclonal antibody (Catalog: HY339010, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.