| Catalog No. | HB941056 |
| Brand | abinScience |
| Host species | Human |
| Tested applications | WB, FCM |
| Species reactivity | Human |
| Form | Liquid |
| Storage buffer | 0.01M PBS pH 7.4. |
| Purity | >95% as determined by SDS-PAGE. |
| Clonality | Monoclonal |
| Isotype | IgG1-kappa-[scFv]2 |
| Applications | ELISA, Bioactivity: FCM, Functional assay, Research in vivo |
| Target | CD279, PD1, PDCD1, Programmed cell death protein 1, Protein PD-1, L-VEGF, N-VEGF, VEGF, VEGFA, VPF, Vascular endothelial growth factor A, long form, Vascular permeability factor |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin level | < 10 EU/mg as determined by LAL method. |
| Accession | Q15116 & P15692 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Alternative name | TetravalentBispecific, AK112, AK-112, AK1122428381-53-5 |
| Note | For research use only. Not suitable for clinical or therapeutic use. |
| Isotype Control | Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507) |
Anti-Human PD-1 & VEGFA Bispecific Reference Antibody (Ivonescimab, RUO)
Overview
Images
 Bioactivity |
Detects VEGF165 in indirect ELISA. |
 Flow CytoMetry |
Flow-cytometry using FITC anti-human VEGFA&PD1 antibody. U87 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human VEGFA&PD1 monoclonal antibody (Catalog: HB941056, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer. |
 Bioactivity |
Detects recombinant human PD1 (Cat No.: HS870021) in indirect ELISA. |
 Western blot |
Western blot analysis was performed using anti-VEGFA/VEGF165 monoclonal antibody at 1ug/mL on various samples. Lane 1: recombinant human VEGFA/VEGF165 (Catalog No: HB941011) Lane 2: negative control |
 Flow cytometry |
Flow-cytometry using anti-human VEGFA antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human VEGFA monoclonal antibody (Catalog HB941056, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 SDS-PAGE |
SDS-PAGE for Research Grade Ivonescimab |