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Anti-Human CD37 Reference Antibody (Ivicentamab, RUO)
Overview
Catalog No. HY564046
Brand abinScience
Host species Human
Tested applications WB, FCM
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS pH 7.4.
Purity >95% purity as determined by SDS-PAGE.
Clonality Monoclonal
Isotype IgG1-kappa
Applications ELISA, Bioactivity: FCM, Functional assay, Research in vivo
Target CD37, Tspan-26, TSPAN26, Leukocyte antigen CD37, Tetraspanin-26
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level < 10 EU/mg as determined by LAL method.
Expression system Mammalian cells
Accession P11049
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative nameDuoHexaBody-CD37, GEN3009, GEN-3009, GEN30092364496-42-2
Note For research use only. Not suitable for clinical or therapeutic use.
Isotype Control Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)
Images


Bioactivity
SEC-HPLC detection for Research Grade Ivicentamab.

SDS-PAGE
SDS-PAGE for Research Grade Ivicentamab.

Flow CytoMetry
Flow-cytometry using APC anti-human CD37 antibody. Raji cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD37 monoclonal antibody (Catalog: HY564046, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Flow CytoMetry
Flow-cytometry using FITC anti-human CD37 antibody. Raji cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD37 monoclonal antibody (Catalog: HY564046, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Flow CytoMetry
Flow-cytometry using PE anti-human CD37 antibody. Raji cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD37 monoclonal antibody (Catalog: HY564046, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Western blot
Western blot analysis was performed using anti-CD37 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CD37 (Catalog No: HY564012)
Lane 2: negative control

Bioactivity
Detects recombinant human CD37 (Catalog No: HY564012) in indirect ELISA