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Anti-Human G-CSFR/CD114 Reference Antibody (Anumigilimab, RUO)
Overview
Catalog No. HV643016
Brand abinScience
Host species Human
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS pH 7.4
Purity >95% purity as determined by SDS-PAGE.
Clonality Monoclonal
Isotype IgG4-kappa
Applications ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Target G-CSF receptor, CSF3R, GCSFR, G-CSF-R, CD114, Granulocyte colony-stimulating factor receptor
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level < 10 EU/mg
Expression system Mammalian cells
Accession Q99062
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative nameCSL324, CSL-324, CSL324, G-CSFR2416593-08-1
Note For research use only. Not suitable for clinical or therapeutic use.
Images


SEC-HPLC		 SEC-HPLC detection for Research Grade Anumigilimab.

Bioactivity		 Detects CD114/CSF3R in indirect ELISAs.

SDS-PAGE		 SDS-PAGE for Research Grade Anumigilimab

Flow CytoMetry		 Flow-cytometry using anti-human CD114 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD114 monoclonal antibody (Catalog: HV643016) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow cytometry		 Flow-cytometry APC using anti-human CD114 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an APC anti-human CD114 monoclonal antibody (Catalog HV643016) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Flow cytometry		 Flow-cytometry PE using anti-human CD114 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an PE anti-human CD114 monoclonal antibody (Catalog HV643016) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Flow cytometry		 Flow-cytometry FITC using anti-human CD114 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an FITC anti-human CD114 monoclonal antibody (Catalog HV643016) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.