| Catalog No. | HC359066 |
| Brand | abinScience |
| Tested applications | FCM |
| Species reactivity | Human |
| Form | Liquid |
| Storage buffer | 0.01M PBS pH 7.4 |
| Purity | >95% purity as determined by SDS-PAGE. |
| Isotype | IgG4-kappa |
| Applications | ELISA, Bioactivity: FACS, Functional assay, Research in vivo |
| Target | Leukocyte surface antigen CD47, CD47, Antigenic surface determinant protein OA3, IAP, Integrin-associated protein, Protein MER6, MER6 |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin level | < 10 EU/mg |
| Expression system | Mammalian cells |
| Accession | Q08722 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Alternative name | AK117, AK-117, AK1172428381-55-7 |
| Note | For research use only. Not suitable for clinical or therapeutic use. |
| Isotype Control | Human IgG4 (S228P) Recombinant Isotype Control Antibody (13R4) (HP298207) Human IgG4 (S228P & L235E) Recombinant Isotype Control Antibody (HyHEL-10) (HP614107) Human IgG4 (S228P) Recombinant Isotype Control Antibody (SAA0184) (HP298107) |
Anti-Human CD47 Reference Antibody (Ligufalimab, RUO)
Overview
Images
 Bioactivity |
Detects Human CD47/MER6 in indirect ELISAs. |
 SDS-PAGE |
SDS-PAGE for Research Grade Ligufalimab. |
 Flow CytoMetry |
Flow-cytometry using PE anti-human CD47 antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD47 monoclonal antibody (Catalog: HC359066, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer. |
 Flow CytoMetry |
Flow-cytometry using FITC anti-human CD47 antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD47 monoclonal antibody (Catalog: HC359066, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer. |
 Flow CytoMetry |
Flow-cytometry using anti-human CD47 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD47 monoclonal antibody (Catalog: HC359066) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer. |