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Endo S2/Endo-beta-N-acetylglucosaminidase
Overview
Catalog No. JN865012
Brand abinScience
Form Lyophilized
Storage buffer Lyophilized from PBS pH7.4, 1 mM EDTA, 4%trehalose, 1% mannitol.
Purity >90% as determined by SDS-PAGE.
Applications Endo S2 hydrolyses N-linked glycans on IgG and α1-acid glycoprotein.
References 1. Nishizawa H, et al. Identification and characterization of a novel thermo-stable endo-β-N-acetylglucosaminidase from Rhizomucorpusillus. J Biosci Bioeng. 2022 Oct;134(4):295-300. [JN865012]
Endotoxin level Please contact with the lab for this information.
Expression system E. coli
Protein length Endo S2/Endo-beta-N-acetylglucosaminidase is cloned from Streptococcus pyogenes and expressed in E.coli.
Nature Recombinant
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Reconstitution Reconstitute in sterile water for a stock solution.
Alternative nameEndo S2, Endo-S2, EndoS2, Endo-beta-N-acetylglucosaminidase
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Note For research use only.
Images


SDS-PAGE
SDS-PAGE for Endo S2/Endo-beta-N-acetylglucosaminidase

Bioactivity
Human IgG1 was incubated with (+) or without (-) Endo S2 and analyzed by reducing SDS–PAGE. A reduced molecular weight of the heavy chain was observed in the Endo S2–treated group.