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Anti-Human CXCR2/IL-8RB Recombinant Nanobody (SAA1360)
Overview
Catalog No. HB243013
Brand abinScience
Host species Alpaca
Tested applications WB, IF
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS pH 7.4.
Purity >95% as determined by SDS-PAGE.
Clonality Monoclonal
Isotype VHH-8His-Cys-tag
Applications ELISA, IF, IP, WB
Target IL8RB, GRO/MGSA receptor, C-X-C chemokine receptor type 2, CD182, CXCR-2, IL-8R B, CXC-R2, IL-8 receptor type 2, High affinity interleukin-8 receptor B, CDw128b, CXCR2
Purification Purified by Nickel column.
Endotoxin level < 10 EU/mg as determined by LAL method.
Accession P25025
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Clone IDSAA1360
Product Usage Information
ApplicationDilution
ELISA 1:2000-1:20000
WB 1:500-1:2000
Note For research use only.
Images


SDS-PAGE
SDS-PAGE for Anti-Human CD182/CXCR2 Nanobody (SAA1360)

Western blot
Western blot analysis was performed using anti-CXCR2 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CXCR2 (Catalog No: HB243012)

Immunofluorescence
Immunofluorescence staining of Hepg2 with Alpaca monoclonal antibody (20ug/ml, overnight at 4°C), followed by Alexa Fluor-488-conjugated secondary antibody (6.5ug/ml, 50minutes at room temperature). The target protein was detected in the Cell membrane.