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Anti-Human 4-1BB/TNFRSF9/CD137 Recombinant Nanobody (SAA1117)
Overview
Catalog No. HC336013
Brand abinScience
Host species Alpaca
Tested applications WB, IF
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS pH 7.4.
Purity >95% as determined by SDS-PAGE.
Clonality Monoclonal
Isotype VHH-8His-Cys-tag
Applications ELISA, WB
Target 4-1BB ligand receptor, CD137, CDw137, ILA, T-cell antigen 4-1BB homolog, T-cell antigen ILA, TNFRSF9, Tumor necrosis factor receptor superfamily member 9
Purification Purified by Nickel column.
Endotoxin level < 10 EU/mg as determined by LAL method.
Accession Q07011
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Clone IDSAA1117
Product Usage Information
ApplicationDilution
ELISA 1:2000-1:20000
WB 1:500-1:2000
Note For research use only.
Images


SDS-PAGE
SDS-PAGE for Anti-Human CD137/TNFRSF9/4-1BB Nanobody (SAA1117)

Bioactivity
Detects recombinant CD137 (Catalog No: HC336011) in indirect ELISA.

Western blot
Western blot analysis was performed using anti-CD137 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CD137 (Catalog No: HC336011)

Immunofluorescence
Immunofluorescence staining of HDLM2 with Alpaca monoclonal antibody (20ug/ml, overnight at 4°C), followed by Alexa Fluor-488-conjugated secondary antibody (6.5ug/ml, 50minutes at room temperature). The target protein was detected in the cell membrane.