Anti-Human PD-1 & PD-L1 Bispecific Reference Antibody (Reozalimab, RUO)

Catalog No.	HV974216
Brand	abinScience
Host species	Human
Tested applications	WB, FCM
Species reactivity	Human
Form	Liquid
Storage buffer	0.01M PBS pH 7.4
Purity	>95% purity as determined by SDS-PAGE.
Clonality	Monoclonal
Isotype	Ig(G1-kappa_G1-lambda2)
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Target	B7-H1, Programmed cell death 1 ligand 1, PDCD1 ligand 1, PDCD1L1, B7 homolog 1, PDCD1LG1, PDL1, hPD-L1, Programmed death ligand 1, B7H1, PD-L1, CD274, Programmed cell death protein 1, Protein PD-1, hPD-1, PD1, PDCD1, CD279
Purification	Protein A/G purified from cell culture supernatant.
Endotoxin level	< 10 EU/mg
Expression system	Mammalian cells
Accession	Q9NZQ7 & Q15116
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative name	Bispecific, IBI318, IBI-318, IBI318, 2445259-99-2
Note	For research use only. Not suitable for clinical or therapeutic use.

Bioactivity	Detects PDL1 in indirect ELISA.
Flow CytoMetry	Flow-cytometry using anti-human CD274 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD274 monoclonal antibody (Catalog: HV974216) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Bioactivity	Detects recombinant human PD1 (Cat No.: HS870021) in indirect ELISA.
Western blot	Western blot analysis was performed using anti-CD274/PD-L1/B7-H1 monoclonal antibody at 1ug/mL on various samples. Lane 1: recombinant human CD274/PD-L1/B7-H1 (Catalog No: HV974011) Lane 2: negative control