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Anti-Human EGFR & ErbB3/Her3 Bispecific Reference Antibody (Izalontamab, RUO)
Overview
Catalog No. HF004206
Brand abinScience
Host species Human
Tested applications FCM
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS pH 7.4.
Purity >95% as determined by SDS-PAGE.
Clonality Monoclonal
Isotype IgG1-kappa-[scFv-heavy-lambda]2
Applications ELISA, Bioactivity: FCM, Functional assay, Research in vivo
Target EC:2.7.10.1, EGFR, ERBB, ERBB1, Epidermal growth factor receptor, HER1, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, EC:2.7.10.1, ERBB3, HER3, Proto-oncogene-like protein c-ErbB-3, Receptor tyrosine-protein kinase erbB-3, Tyrosine kinase-type cell surface receptor HER3
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level < 10 EU/mg as determined by LAL method.
Accession P00533 & P21860
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative nameTetravalentbispecific, SI-1, SI-1X6.4, SI-B001, 2559704-24-2
Note For research use only. Not suitable for clinical or therapeutic use.
Isotype Control Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)
Images


Bioactivity
Detects EGFR in indirect ELISA.

Flow cytometry
Flow-cytometry using anti-human ERBB3 antibody. HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human ERBB3 monoclonal antibody (Catalog HF004206, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.