InVivoMAb Anti-Human CD40/TNFRSF5 Antibody (Iv0136)

Catalog No.

HB782010

Brand

abinScience

Host species

Human

Tested applications

FCM

Species reactivity

Human

Form

Liquid

Storage buffer

0.01M PBS pH 7.4.

Purity

>95% as determined by SDS-PAGE.

Clonality

Monoclonal

Isotype

IgG1, kappa

Applications

ELISA, Neutralization, FCM

Target

B-cell surface antigen CD40, Bp50, CD40, CD40L receptor, CDw40, TNFRSF5, Tumor necrosis factor receptor superfamily member 5

Purification

Protein A/G purified from cell culture supernatant.

Endotoxin level

< 1 EU/mg as determined by LAL method.

Accession

P25942

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Clone ID

Iv0136

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Note

For research use only. Not suitable for clinical or therapeutic use.

Isotype Control

Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)

Bioactivity	Detects CD40/TNFRSF5 in indirect ELISAs.
SDS-PAGE	SDS-PAGE for InVivoMAb Anti-Human CD40/TNFRSF5 Antibody (Iv0136).
Flow CytoMetry	Flow-cytometry using APC anti-human CD40 antibody. U2OS cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD40 monoclonal antibody (Catalog: HB782010, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry	Flow-cytometry using FITC anti-human CD40 antibody. U2OS cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD40 monoclonal antibody (Catalog: HB782010, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.