InVivoMAb Anti-Human 4-1BB/TNFRSF9/CD137 Antibody (Iv0138)

Catalog No.

HC336020

Brand

abinScience

Host species

Human

Tested applications

FCM

Species reactivity

Human

Form

Liquid

Storage buffer

0.01M PBS pH 7.4.

Purity

>95% as determined by SDS-PAGE.

Clonality

Monoclonal

Isotype

IgG1, kappa

Applications

ELISA, FCM, Neutralization

Target

4-1BB ligand receptor, CD137, CDw137, ILA, T-cell antigen 4-1BB homolog, T-cell antigen ILA, TNFRSF9, Tumor necrosis factor receptor superfamily member 9

Purification

Protein A/G purified from cell culture supernatant.

Endotoxin level

< 1 EU/mg as determined by LAL method.

Accession

Q07011

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Clone ID

Iv0138

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Note

For research use only. Not suitable for clinical or therapeutic use.

Isotype Control

Anti-LASV GPC Recombinant Antibody (Fab 37.7) (VK453013)

Bioactivity	Detects CD137/TNFRSF9/4-1BB in indirect ELISAs.
SDS-PAGE	SDS-PAGE for InVivoMAb Anti-Human CD137/TNFRSF9/4-1BB Antibody (Iv0138).
Flow CytoMetry	Flow-cytometry using PE anti-human CD137 antibody. HDLM-2 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD137 monoclonal antibody (Catalog: HC336020, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Immunofluorescence	Immunofluorescence staining of HDLM2 with Human monoclonal antibody (20ug/ml, overnight at 4°C), followed by Alexa Fluor-488-conjugated secondary antibody (6.5ug/ml, 50minutes at room temperature). The target protein was detected in the cell membrane.