| Catalog No. | MB941013 | ||||
| Brand | abinScience | ||||
| Host species | Mouse | ||||
| Tested applications | WB, FCM | ||||
| Species reactivity | Human, Mouse, Rat, Rabbit | ||||
| Form | Liquid | ||||
| Storage buffer | 0.01M PBS pH 7.4. | ||||
| Purity | >95% as determined by SDS-PAGE. | ||||
| Clonality | Monoclonal | ||||
| Isotype | IgG2a, kappa | ||||
| Applications | Blocking, ELISA, FCM | ||||
| Target | L-VEGF, N-VEGF, VEGF, VEGFA, VPF, Vascular endothelial growth factor A, long form, Vascular permeability factor | ||||
| Purification | Protein A/G purified from cell culture supernatant. | ||||
| Endotoxin level | < 10 EU/mg | ||||
| Accession | Q00731 | ||||
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||
| Clone ID | G6 | ||||
| Product Usage Information |
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| Note | For research use only. | ||||
| RRID | Anti-Mouse VEGFA Antibody (G6) (abinScience Cat# MB941013, RRID:AB_3727197) |
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| Isotype Control | Mouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023) |
Anti-Mouse VEGFA Recombinant Antibody (G6)
Overview
Images
 Bioactivity |
Detects Mouse VEGFA in indirect ELISAs. |
 SDS-PAGE |
SDS-PAGE for Anti-Mouse VEGFA Antibody (G6) |
 Flow CytoMetry |
Flow-cytometry using FITC anti-human VEGF antibody. U87 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human VEGF monoclonal antibody (Catalog: MB941013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer. |
 Western blot |
Western blot analysis was performed using anti-VEGFA/VEGF165 monoclonal antibody at 1ug/mL on various samples. Lane 1: recombinant human VEGFA/VEGF165 (Catalog No: HB941011) Lane 2: negative control |