| Catalog No. | RB941013 | ||||
| Brand | abinScience | ||||
| Tested applications | WB, FCM | ||||
| Species reactivity | Human, Mouse, Rat, Rabbit | ||||
| Form | Liquid | ||||
| Storage buffer | 0.01M PBS pH 7.4 | ||||
| Purity | >95% as determined by SDS-PAGE. | ||||
| Isotype | IgG2a, kappa | ||||
| Applications | Blocking, ELISA | ||||
| Target | Vascular endothelial growth factor A, VPF, VEGFA, VEGF, Vascular permeability factor, VEGF-A | ||||
| Purification | Protein A/G purified from cell culture supernatant. | ||||
| Endotoxin level | Please contact with the lab for this information. | ||||
| Accession | P16612 | ||||
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | ||||
| Clone ID | B20-4 | ||||
| Product Usage Information |
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| Note | For research use only. | ||||
| RRID | Anti-Rat VEGFA Antibody (B20-4) (abinScience Cat# RB941013, RRID:AB_3727198) |
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| Isotype Control | Mouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023) |
Anti-Rat VEGF Recombinant Antibody (B20-4)
Overview
Images
 Bioactivity |
Detects Rat VEGFA in indirect ELISAs. |
 SDS-PAGE |
SDS-PAGE for Anti-Rat VEGFA Antibody (B20-4). |
 Flow CytoMetry |
Flow-cytometry using FITC anti-human VEGF antibody. U87 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human VEGF monoclonal antibody (Catalog: RB941013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer. |
 Flow CytoMetry |
Flow-cytometry using PE anti-human VEGF antibody. U87 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human VEGF monoclonal antibody (Catalog: RB941013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer. |
 Western blot |
Western blot analysis was performed using anti-VEGFA/VEGF165 monoclonal antibody at 1ug/mL on various samples. Lane 1: recombinant human VEGFA/VEGF165 (Catalog No: HB941011) Lane 2: negative control |