Anti-Rat VEGFA Recombinant Antibody (B20-4)

Catalog No.

RB941013

Brand

abinScience

Host species

Mouse

Tested applications

WB, FCM

Species reactivity

Human, Mouse, Rat, Rabbit

Form

Liquid

Storage buffer

0.01M PBS pH 7.4.

Purity

>95% as determined by SDS-PAGE.

Clonality

Monoclonal

Isotype

IgG2a, kappa

Applications

Blocking, ELISA

Target

L-VEGF, N-VEGF, VEGF, VEGFA, VPF, Vascular endothelial growth factor A, long form, Vascular permeability factor

Purification

Protein A/G purified from cell culture supernatant.

Endotoxin level

< 10 EU/mg

Accession

P16612

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Clone ID

B20-4

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Note

For research use only.

RRID

Anti-Rat VEGFA Antibody (B20-4) (abinScience Cat# RB941013, RRID:AB_3727198)

Isotype Control

Mouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023)

Bioactivity	Detects Rat VEGFA in indirect ELISAs.
SDS-PAGE	SDS-PAGE for Anti-Rat VEGFA Antibody (B20-4).
Flow CytoMetry	Flow-cytometry using FITC anti-human VEGF antibody. U87 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human VEGF monoclonal antibody (Catalog: RB941013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry	Flow-cytometry using PE anti-human VEGF antibody. U87 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human VEGF monoclonal antibody (Catalog: RB941013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Western blot	Western blot analysis was performed using anti-VEGFA/VEGF165 monoclonal antibody at 1ug/mL on various samples. Lane 1: recombinant human VEGFA/VEGF165 (Catalog No: HB941011) Lane 2: negative control