Anti-Human IgE/IGHE Recombinant Antibody (SAA0343)

Catalog No.

HV064033

Brand

abinScience

Host species

Mouse

Tested applications

FCM

Species reactivity

Human

Form

Liquid

Storage buffer

0.01M PBS pH 7.4.

Purity

>95% as determined by SDS-PAGE.

Clonality

Monoclonal

Isotype

IgG2a, kappa

Applications

ELISA, FCM

Target

IGHE, Ig epsilon chain C region, Ig epsilon chain C region ND, Immunoglobulin heavy constant epsilon

Purification

Protein A/G purified from cell culture supernatant.

Endotoxin level

< 10 EU/mg

Accession

P01854

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Clone ID

SAA0343

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Note

For research use only.

Isotype Control

Mouse IgG2a Recombinant Isotype Control Antibody (S43.10) (MF533307)

Flow Cytometry	Flow-cytometry using anti-human IgE antibody.IgE Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IgE antibody monoclonal antibody (Catalog # HV064033 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry	Flow-cytometry using anti-human IgE antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human IgE antibody monoclonal antibody (Catalog # HV064033 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE	SDS-PAGE for Anti-Human IgE/IGHE Antibody (SAA0343).