Anti-Human VISTA/B7-H5/PD-1H Reference Antibody (HMBD-002, RUO)

Catalog No.	HP543036
Brand	abinScience
Tested applications	FCM
Species reactivity	Human
Form	Liquid
Storage buffer	0.01M PBS pH 7.4
Purity	>95% purity as determined by SDS-PAGE.
Isotype	IgG4, kappa
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Target	V-set domain-containing immunoregulatory receptor, C10orf54, VSIR, VISTA, SISP1, Stress-induced secreted protein-1, Platelet receptor Gi24, V-set immunoregulatory receptor, Sisp-1, V-type immunoglobulin domain-containing suppressor of T-cell activation
Purification	Protein A/G purified from cell culture supernatant.
Endotoxin level	< 10 EU/mg
Expression system	Mammalian cells
Accession	Q9H7M9
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Clone ID	HMBD-002
Note	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Human IgG4 (S228P & L235E) Recombinant Isotype Control Antibody (HyHEL-10) (HP614107)

Bioactivity	Detects Human VSIR/VISTA in indirect ELISAs.
SDS-PAGE	SDS-PAGE for Research Grade Anti-Human VSIR/VISTA Antibody (HMBD-002).
Flow cytometry	Flow-cytometry APC using anti-human VSIR antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an APC anti-human VSIR monoclonal antibody (Catalog HP543036) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry	Flow-cytometry FITC using anti-human VSIR antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an FITC anti-human VSIR monoclonal antibody (Catalog HP543036) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.