Anti-Human MICA/B Reference Antibody (IPH4301, RUO)

Catalog No.

HS894016

Brand

abinScience

Host species

Human

Species reactivity

Human

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Purity

>95% purity as determined by SDS-PAGE.

Clonality

Monoclonal

Isotype

IgG1-kappa

Applications

ELISA, Bioactivity: FACS, Functional assay, Research in vivo

Target

MICA, MIC-A, MHC class I polypeptide-related sequence A, PERB11.1, MHC class I polypeptide-related sequence B, MIC-B, MICB, PERB11.2

References

1. Morel A, et al. Abstract 1491: IPH4301, an antibody targeting MICA and MICB exhibits potent cytotoxic activity and immunomodulatory properties for the treatment of cancer. Cancer Res. 2016 Jul;76(14 Suppl):1491. [HS894016]
2. Wang Z, et al. Preliminary study on PEG-G5.NH2-FITC-DOTA(Gd)-Monalizumab/IPH4301 probe. Biotechnol Bull. 2023;10. [HS894016]

Purification

Protein A/G purified from cell culture supernatant.

Biological activity

IPH4301 induces death of tumor cells expressing MICA/B in vitro via antibody-dependent cellular cytotoxicity and antibody-dependent cellular phagocytosis [1]. IPH4301 blocks the binding of MICA/B to NKG2D, and restores the expression and function of NKG2D on primary human NK cells and T cells [1]. IPH4301 reverses the immunosuppressive effect induced by M2 macrophages and restores the ADCC activity of autologous NK cells against MICA-expressing tumor cell lines [1]. IPH4301-derived PEG-G5.NH2-FITC-DOTA(Gd)-Monalizumab/IPH4301 nanoprobe can efficiently bind to NK-92MI cells. Its binding efficiency and in vitro MRI signal increase with elevated concentrations; besides, it upregulates IFN-γ secretion of NK-92MI cells, enhances the cytotoxicity against MDA-MB-231 triple-negative breast cancer cells and induces tumor cell apoptosis [2].

Endotoxin level

< 10 EU/mg

Expression system

Mammalian cells

Accession

Q29983, Q29980

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternative name

IPH4301

Clone ID

IPH4301

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Note

For research use only. Not suitable for clinical or therapeutic use.

Bioactivity	Detects MICA in indirect ELISAs.
SDS-PAGE	SDS-PAGE for Research Grade IPH4301.