Anti-HRSV Post-Fusion Glycoprotein Reference Antibody (ADI-14359, RUO)

Catalog No.	VK421116
Brand	abinScience
Host species	Human
Species reactivity	Human respiratory syncytial virus A (HRSV-A)
Form	Liquid
Storage buffer	0.01M PBS pH 7.4
Purity	>95% purity as determined by SDS-PAGE.
Clonality	Monoclonal
Isotype	IgG1, kappa
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Target	F, Fusion glycoprotein F0, Fusion glycoprotein F2, p27, Intervening segment, Pep27, Peptide 27, Fusion glycoprotein F1
References	1. Goodwin E, et al. Infants Infected with Respiratory Syncytial Virus Generate Potent Neutralizing Antibodies that Lack Somatic Hypermutation. Immunity. 2018;48(2):339-349.e5. [VK421116]
Purification	Protein A/G purified from cell culture supernatant.
Biological activity	ADI-14359 binds to epitope I of RSV postF via convergent CDR H3 motifs and germline-encoded features of its IGKV1-39 light chain and IGHV2-70 heavy chain, with key interactions mediated by specific hydrogen bonds and electrostatic contacts [1]. ADI-14359 (6.25 nM-1600 nM) binds to RSV postF with a K D value of 387 nM; the heavy chain Arg50 residue and postF Lys390 residue are critical for this interaction, as disruption of these residues significantly reduces or even completely abolishes binding [1]. ADI-14359 recognizes antigenic epitope I on RSV F, preferentially binds to the post-fusion conformation, and does not neutralize RSV [1].
Endotoxin level	< 10 EU/mg
Expression system	Mammalian cells
Accession	P03420
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative name	ADI-14359
Clone ID	ADI-14359
Note	For research use only. Not suitable for clinical or therapeutic use.