Anti-Human 4-1BB Ligand/TNFSF9 Reference Antibody (CTX-471, RUO)

Catalog No.	HC336036
Brand	abinScience
Host species	Human
Species reactivity	Human
Form	Liquid
Storage buffer	0.01M PBS pH 7.4
Purity	>95% purity as determined by SDS-PAGE.
Clonality	Monoclonal
Isotype	IgG
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Target	Tumor necrosis factor receptor superfamily member 9, CD137, 4-1BB ligand receptor, CDw137, TNFRSF9, T-cell antigen ILA, T-cell antigen 4-1BB homolog, ILA
References	1. Ugur Eskiocak, et al. Differentiated agonistic antibody targeting CD137 eradicates large tumors without hepatotoxicity. JCI Insight. 2020 Mar 12;5(5):e133647. [HC336036]
Purification	Protein A/G purified from cell culture supernatant.
Biological activity	CTX-471 (5-500 nM) has bind affinity for recombinant human, cynomolgus macaque CD137 and mouse CD137 with K d values of 50 nM, 61 nM and 748 nM, respectively [1]. CTX-471 binds to a unique epitope on CD137 [1]. CTX-471 (0.1-100 nM; 1, 10 ug/mL; 3 days) increases IFN-y production by human T cells in an Fcy receptor-dependent (FcyR-dependent) manner in vitro [1]. CTX-471 (i.p.; 150 ug) exhibits curative monotherapy activity in various syngeneic tumor models and shows a unique ability to cure mice of very large tumors. CTX-471 (i.v.; 10-80 mg/kg; on days 0, 7, 14, and 21) is well tolerated, with no signs of hepatic toxicity in high doses.
Endotoxin level	< 10 EU/mg
Expression system	Mammalian cells
Accession	Q07011
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative name	CTX-471, CTX471, CTX471
Clone ID	CTX-471
Note	For research use only. Not suitable for clinical or therapeutic use.