| Catalog No. | HW310023 | ||||||
| Brand | abinScience | ||||||
| Tested applications | WB, FCM | ||||||
| Species reactivity | Human, Mouse | ||||||
| Form | Liquid | ||||||
| Storage buffer | 0.01M PBS pH 7.4 | ||||||
| Purity | >95% as determined by SDS-PAGE. | ||||||
| Isotype | IgG1, kappa | ||||||
| Applications | ELISA, FCM, WB | ||||||
| Target | GPC3, MXR7, GTR2-2, Glypican-3, Intestinal protein OCI-5, OCI5 | ||||||
| Purification | Protein A/G purified from cell culture supernatant. | ||||||
| Endotoxin level | Please contact with the lab for this information. | ||||||
| Accession | P51654 | ||||||
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | ||||||
| Clone ID | YP7 | ||||||
| Product Usage Information |
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| Note | For research use only. | ||||||
| RRID | Anti-Human GPC3 Antibody (YP7) (abinScience Cat# HW310023, RRID:AB_3727765) |
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| Isotype Control | Human IgG1 (LALA) Recombinant Isotype Control Antibody (HyHEL-10) (HP688107) |
Anti-Human Glypican 3 Recombinant Antibody (YP7)
Overview
Images
 Bioactivity |
Detects Human GPC3 in indirect ELISAs. |
 Flow Cytometry |
Flow-cytometry using anti-human GPC3 antibody.GPC3 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human GPC3 antibody monoclonal antibody (Catalog # HW310023 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 Flow Cytometry |
Flow-cytometry using anti-human GPC3 antibody.HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human GPC3 antibody monoclonal antibody (Catalog # HW310023 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 Flow Cytometry |
Flow-cytometry using anti-human GPC3 antibody.Human Jurkat cell line were stained with an irrelevant antibody (Blue Histogram) or an anti-human GPC3 antibody monoclonal antibody (Catalog # HW310023 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 SDS-PAGE |
SDS-PAGE for Anti-Human GPC3 Antibody (YP7). |
 Flow CytoMetry |
Flow-cytometry using anti-human GPC3 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human GPC3 monoclonal antibody (Catalog: HW310023) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 Western blot |
Western blot analysis was performed using anti-GPC3/Glypican 3 monoclonal antibody at 1µg/ml on various samples. Lane 1: recombinant mouse GPC3/Glypican 3 (Catalog No: MW310011) Lane 2: negative control |
 Western blot |
Western blot analysis was performed using anti-GPC3 monoclonal antibody at 1ug/mL on various samples. Lane 1: HepG2 cell lysate |