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Anti-GFP-plus Tag Recombinant Antibody (SAA0408)
Overview
Catalog No. ZP140013
Brand abinScience
Host species Mouse
Tested applications IF
Species reactivity General
Form Liquid
Storage buffer 0.01M PBS pH 7.4.
Purity >95% as determined by SDS-PAGE.
Clonality Monoclonal
Isotype VHH-mFc
Applications ELISA, IF, WB
Target Green fluorescent protein, GFP, Tag Antibody
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level < 10 EU/mg as determined by LAL method.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone IDSAA0408
Product Usage Information
ApplicationDilution
ELISA 1:2000-1:20000
WB 1:500-1:2000
Note For research use only.
Images


SDS-PAGE
SDS-PAGE for Anti-GFP-plus® Tag Antibody (SAA0408)

Immunofluorescence
Immunofluorescent analysis of 4% PFA-fixed and 0.1% Triton X-100-permeabilized 293T cells transfected with pEGFP-N1 using GFP antibody (Cat. No. YP144013) at 20 μg/mL. CY3-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) was used as the secondary antibody, and nuclei were counterstained with DAPI (blue).