| Catalog No. | HF879053 | ||||||
| Brand | abinScience | ||||||
| Host species | Human | ||||||
| Tested applications | FCM | ||||||
| Species reactivity | Human | ||||||
| Form | Liquid | ||||||
| Storage buffer | 0.01M PBS pH 7.4. | ||||||
| Purity | >95% as determined by SDS-PAGE. | ||||||
| Clonality | Monoclonal | ||||||
| Isotype | IgG1, kappa | ||||||
| Applications | ELISA, FCM, WB | ||||||
| Target | C-domain 1, C-domain 2, Cachectin, ICD1, ICD2, Intracellular domain 1, Intracellular domain 2, N-terminal fragment, NTF, TNF, TNF-a, TNF-alpha, TNFA, TNFSF2, Tumor necrosis factor, Tumor necrosis factor ligand superfamily member 2, Tumor necrosis factor, membrane form, Tumor necrosis factor, soluble form | ||||||
| Purification | Protein A/G purified from cell culture supernatant. | ||||||
| Endotoxin level | < 10 EU/mg | ||||||
| Accession | P01375 | ||||||
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||
| Clone ID | SAA2005 | ||||||
| Product Usage Information |
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| Note | For research use only. | ||||||
| RRID | Anti-Human TNFa/TNF-alpha Antibody (SAA2005) (abinScience Cat# HF879053, RRID:AB_3727917) |
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| Isotype Control | Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507) |
Anti-Human TNF-alpha Recombinant Antibody (SAA2005)
Overview
Images
 Flow Cytometry |
Flow-cytometry using anti-human TNFa antibody.TNFa Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human TNFa antibody monoclonal antibody (Catalog # HF879053 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 SDS-PAGE |
SDS-PAGE for Anti-Human TNFa/TNF-alpha Antibody (SAA2005). |