| Catalog No. | HB782023 | ||||||
| Brand | abinScience | ||||||
| Host species | Human | ||||||
| Tested applications | FCM | ||||||
| Species reactivity | Human | ||||||
| Form | Liquid | ||||||
| Storage buffer | 0.01M PBS pH 7.4. | ||||||
| Purity | >95% as determined by SDS-PAGE. | ||||||
| Clonality | Monoclonal | ||||||
| Isotype | IgG1, kappa | ||||||
| Applications | ELISA, WB, FCM | ||||||
| Target | B-cell surface antigen CD40, Bp50, CD40, CD40L receptor, CDw40, TNFRSF5, Tumor necrosis factor receptor superfamily member 5 | ||||||
| Purification | Protein A/G purified from cell culture supernatant. | ||||||
| Endotoxin level | < 10 EU/mg | ||||||
| Accession | P25942 | ||||||
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||
| Clone ID | SAA2006 | ||||||
| Product Usage Information |
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| Note | For research use only. | ||||||
| RRID | Anti-Human CD40/TNFRSF5 Antibody (SAA2006) (abinScience Cat# HB782023, RRID:AB_3727918) |
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| Isotype Control | Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507) |
Anti-Human CD40/TNFRSF5 Recombinant Antibody (SAA2006)
Overview
Images
 Bioactivity |
SEC-HPLC detection for Anti-Human CD40/TNFRSF5 Antibody (ABS2001). |
 Flow Cytometry |
Flow-cytometry using anti-human CD40 antibody. U2OS cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD40 monoclonal antibody (Catalog HB782023, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 SDS-PAGE |
SDS-PAGE for Anti-Human CD40/TNFRSF5 Antibody (SAA2006). |
 Flow CytoMetry |
Flow-cytometry using APC anti-human CD40 antibody. U2OS cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD40 monoclonal antibody (Catalog: HB782023, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer. |
 Flow CytoMetry |
Flow-cytometry using PE anti-human CD40 antibody. U2OS cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD40 monoclonal antibody (Catalog: HB782023, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer. |