MAPT, MAPTL, MTBT1, Microtubule-associated protein tau, Neurofibrillary tangle protein, PHF-tau, Paired helical filament-tau, TAU
Purification
Protein A/G purified from cell culture supernatant.
Endotoxin level
< 10 EU/mg
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Flow-cytometry using FITC anti-human MAPT antibody. HDLM-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human MAPT monoclonal antibody (Catalog: HY086193, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry
Flow-cytometry using APC anti-human MAPT antibody. HDLM-2 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human MAPT monoclonal antibody (Catalog: HY086193, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry
Flow-cytometry using PE anti-human MAPT antibody. HDLM-2 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human MAPT monoclonal antibody (Catalog: HY086193, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.