Anti-Human ASGR1/ASGPR1 Reference Antibody (AMG 529, RUO)

Catalog No.	HY525016
Brand	abinScience
Species reactivity	Human
Form	Liquid
Storage buffer	0.01M PBS pH 7.4
Purity	>95% purity as determined by SDS-PAGE.
Isotype	IgG1-kappa
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Target	HL-1, ASGP-R 1, Asialoglycoprotein receptor 1, CLEC4H1, ASGR1, C-type lectin domain family 4 member H1, ASGPR 1, Hepatic lectin H1
References	1. Janiszewski M, et al. A randomized, placebo-controlled, double-blind, ascending single-dose, phase 1 study to evaluate the safety, tolerability, pharmacokinetics, and pharmacodynamics of AMG 529, a novel anti-ASGR1 monoclonal antibody, in healthy subjects. Journal of the American College of Cardiology. 2019 Mar 12;73(9S1):1755-. [HY525016]
Purification	Protein A/G purified from cell culture supernatant.
Biological activity	AMG-529 induces a dose-dependent increase in alkaline phosphatase (ALP) levels, but no significant effect on blood lipid levels (including non-high-density lipoprotein cholesterol) is observed [1].
Endotoxin level	< 10 EU/mg
Expression system	Mammalian cells
Accession	P07306
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative name	AMG-529, AMG529, AMG529
Clone ID	AMG 529
Note	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Human IgG1 (LALAPG) Recombinant Isotype Control Antibody (HyHEL-10) (HP688307)

SDS-PAGE	SDS-PAGE for Research Grade Anti-Human ASGR1 (AMG 529).
Flow CytoMetry	Flow-cytometry using APC anti-human ASGR1 antibody. HepG-2 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human ASGR1 monoclonal antibody (Catalog: HY525016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry	Flow-cytometry using FITC anti-human ASGR1 antibody. HepG-2 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human ASGR1 monoclonal antibody (Catalog: HY525016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry	Flow-cytometry using PE anti-human ASGR1 antibody. HepG-2 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human ASGR1 monoclonal antibody (Catalog: HY525016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry	Flow-cytometry using anti-human ASGR1 antibody. HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human ASGR1 monoclonal antibody (Catalog HY525016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.