Anti-Human B7-H4 Reference Antibody (NC762, RUO)

Catalog No.	HV721026
Brand	abinScience
Species reactivity	Human
Form	Liquid
Storage buffer	0.01M PBS pH 7.4
Purity	>95% purity as determined by SDS-PAGE.
Isotype	IgG1-kappa
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Target	V-set domain-containing T-cell activation inhibitor 1, B7h.5, B7 homolog 4, B7-H4, T-cell costimulatory molecule B7x, B7H4, VTCN1, Protein B7S1, Immune costimulatory protein B7-H4
References	1. Myint, Han, et al. "748 A phase 1/2, open-label, dose-escalation, safety and tolerability study of NC762 in subjects with advanced or metastatic solid tumors." (2022). [HV721026] 2. Kristina Archer, et al. Abstract 3193: Development and functional characterization of NC762, a novel therapeutic antibody targeting B7-H4, for the treatment of malignancies. Cancer Res 1 July 2021; 81 (13_Supplement): 3193. [HV721026]
Purification	Protein A/G purified from cell culture supernatant.
Biological activity	NC762 (0.01-100 nM) binds to B7-H4 with an EC 50 of 1.12 nM in 624Mel.B7H4 cells and EC 50 of 0.976 nM in SKBR3 cells [2]. NC762 (0.1 ng/mL-100 ug/mL) binds to B7-H4 in human 293T cells but not mouse B7-H4 [2]. NC762 (0.1 pM-1000 nM) suggests increased binding to CD16a compared to V5.hlgG 1 [2]. NC762 (0.1-100 pM, 5 h) exhibits ADCC activity in 624Mel.B7H4 and SKBR3 cultured with effector Jurkat reporter cell line [2]. through binding to tumor-expressing B7-H4. NC762 can be studies in research on cancer such as advanced or metastatic solid tumors [1].
Endotoxin level	< 10 EU/mg
Expression system	Mammalian cells
Accession	Q7Z7D3
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative name	NC762
Clone ID	NC762
Note	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)