| Catalog No. | HC654016 |
| Brand | abinScience |
| Host species | Human |
| Species reactivity | Human |
| Form | Liquid |
| Storage buffer | 0.01M PBS pH 7.4 |
| Purity | >95% purity as determined by SDS-PAGE. |
| Clonality | Monoclonal |
| Isotype | IgG |
| Applications | ELISA, Bioactivity: FACS, Functional assay, Research in vivo |
| Target | ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2, Bone marrow stromal antigen 1, BST-1, ADP-ribosyl cyclase 2, CD157, Cyclic ADP-ribose hydrolase 2, BST1, cADPr hydrolase 2 |
| References | 1. Aud D, et al. MEN1112, a Novel Humanized De-Fucosylated Monoclonal Antibody with High Affinity and Specificity for Bst1/CD157 Antigen and Enhanced CD16 Binding. Blood. 2014 Dec 6;124(21):3607. [HC654016] 2. Venditti A, et al. MEN1112/OBT357, an Anti Bst1/CD157 Humanized Antibody Inducing Acute Myelogenous Leukemia (AML) Blast Depletion in an Autologous Ex Vivo Assay: A Potential New Targeted Therapy for AML. Blood. 2015;126(23):788. [HC654016] 3. Krupka C, et al. Targeting AML Using an Fc-Engineered BST1/CD157 Monoclonal Antibody. Blood. 2014 Dec 6;124(21):987. [HC654016] |
| Purification | Protein A/G purified from cell culture supernatant. |
| Biological activity | MEN1112 specifically binds to Bst1/CD157-positive cell lines with an EC 50 of 1 nM, and does not bind to Bst1/CD157-negative cell lines [1]. MEN1112 binds to recombinant Bst1/CD157 antigen with an EC 50 of 0.4 nM [1]. MEN1112 exhibits stronger high-affinity binding to both high- and low-affinity alleles of the CD16A Fc receptor on effector cells due to its defucosylated Fc domain [1]. MEN1112 induces slow internalization of the Bst1/CD157 antigen on target cells, a property that helps enhance its ADCC efficacy [1]. MEN1112 potently mediates antibody-dependent cell-mediated cytotoxicity (ADCC) against U937 acute myeloid leukemia (AML) cells, with an EC 50 of 30-140 pM [3]. MEN1112-mediated target cell lysis (20 h post-administration) is significantly enhanced upon the addition of 5% healthy donor serum to the co-culture system of healthy monocytes and autologous NK cells [3]. |
| Endotoxin level | < 10 EU/mg |
| Expression system | Mammalian cells |
| Accession | Q10588 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Alternative name | MEN1112 |
| Clone ID | MEN1112 |
| Note | For research use only. Not suitable for clinical or therapeutic use. |
Anti-Human CD157 Reference Antibody (MEN1112, RUO)
Overview
Images
 SDS-PAGE |
SDS-PAGE for Research Grade Anti-Human CD157/BST1 (MEN1112) |