Anti-GFP Recombinant Nanobody (SAA0958)
Overview
| Catalog No. |
ZP144033 |
| Brand |
abinScience |
| Host species |
Alpaca |
| Species reactivity |
General |
| Form |
Liquid |
| Storage buffer |
0.01M PBS pH 7.4 |
| Purity |
>95% as determined by SDS-PAGE. |
| Clonality |
Monoclonal |
| Isotype |
VHH-8His-Cys-tag |
| Applications |
ELISA, IF |
| Target |
Green fluorescent protein, GFP |
| Purification |
Purified by Nickel column |
| Endotoxin level |
Please contact with the lab for this information. |
| Stability and Storage |
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Reconstitution |
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Clone ID | SAA0958 |
| Product Usage Information |
| Application | Dilution |
|---|
| ELISA | 1:2000-1:20000 |
|
| Note |
For research use only. |
Images
 SDS-PAGE |
SDS-PAGE for Anti-GFP Nanobody (SAA0958) |
 Immunofluorescence |
Immunofluorescent analysis of 4% PFA-fixed and 0.1% Triton X-100-permeabilized 293T cells transfected with pEGFP-N1 using VHH anti-GFP antibody (Cat. No. ZP144033, His-tagged) at 20 μg/mL. After incubation with mouse anti-His antibody, CY3-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue). |