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Anti-GFP Recombinant Nanobody (SAA0958)
Overview
Catalog No. ZP144033
Brand abinScience
Host species Alpaca
Tested applications IF
Species reactivity General
Form Liquid
Storage buffer 0.01M PBS pH 7.4.
Purity >95% as determined by SDS-PAGE.
Clonality Monoclonal
Isotype VHH-8His-Cys-tag
Applications ELISA, IF
Target Green fluorescent protein, GFP
Purification Purified by Nickel column.
Endotoxin level < 10 EU/mg as determined by LAL method.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Clone IDSAA0958
Product Usage Information
ApplicationDilution
ELISA 1:2000-1:20000
Note For research use only.
Images


SDS-PAGE
SDS-PAGE for Anti-GFP Nanobody (SAA0958)

Immunofluorescence
Immunofluorescent analysis of 4% PFA-fixed and 0.1% Triton X-100-permeabilized 293T cells transfected with pEGFP-N1 using VHH anti-GFP antibody (Cat. No. ZP144033, His-tagged) at 20 μg/mL. After incubation with mouse anti-His antibody, CY3-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).