Anti-Human EMMPRIN/CD147 Recombinant Antibody (4A5)

Catalog No.

HW664023

Brand

abinScience

Tested applications

WB, FCM

Species reactivity

Human

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Purity

>95% as determined by SDS-PAGE.

Isotype

IgG2a, kappa

Applications

Target

Hepatoma-associated antigen, BSG, HAb18G, 5F7, TCSF, OK blood group antigen, Collagenase stimulatory factor, CD147, Leukocyte activation antigen M6, Basigin, Extracellular matrix metalloproteinase inducer, EMMPRIN, Tumor cell-derived collagenase stimulatory factor

Purification

Protein A/G purified from cell culture supernatant.

Endotoxin level

Please contact with the lab for this information.

Accession

P35613

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

4A5

Product Usage Information

Application	Dilution
WB	1:500-1:2000

Note

For research use only.

RRID

Anti-Human CD147/BSG Antibody (4A5) (abinScience Cat# HW664023, RRID:AB_3728962)
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Isotype Control

Mouse IgG2a, kappa Recombinant Isotype Control Antibody (MOPC-173) (MF533507)
Mouse IgG2a Recombinant Isotype Control Antibody (S43.10) (MF533307)

Bioactivity	Detects Human CD147/BSG in indirect ELISAs.
SDS-PAGE	SDS-PAGE for Anti-Human CD147/BSG Antibody (4A5).
Flow CytoMetry	Flow-cytometry using FITC anti-human CD147 antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD147 monoclonal antibody (Catalog: HW664023, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Western blot	Western blot analysis was performed using anti-CD147/BSG monoclonal antibody at 1μg/ml on various samples. Lane 1: recombinant human CD147/BSG (Catalog No: HW664011)