Anti-Human HRAS Recombinant Antibody (Y13-259)

Catalog No.

HF778023

Brand

abinScience

Species reactivity

Human

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Purity

>95% as determined by SDS-PAGE.

Isotype

IgG2a, kappa

Applications

IF, IP, WB

Target

c-H-ras, GTPase HRas, H-Ras-1, p21ras, Transforming protein p21, Ha-Ras, HRAS1, HRAS

Purification

Protein A/G purified from cell culture supernatant.

Endotoxin level

Please contact with the lab for this information.

Accession

P01112

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

Y13-259

Product Usage Information

Application	Dilution
WB	1:500-1:2000

Note

For research use only.

RRID

Anti-Human HRAS Antibody (Y13-259) (abinScience Cat# HF778023, RRID:AB_3728964)
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Isotype Control

Mouse IgG2a Recombinant Isotype Control Antibody (S43.10) (MF533307)

SDS-PAGE	SDS-PAGE for Anti-Human HRAS Antibody (Y13-259).
Flow CytoMetry	Flow-cytometry using FITC anti-human HRAS antibody. MCF-7 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human HRAS monoclonal antibody (Catalog: HF778023, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Western blot	Western blot analysis was performed using anti-KRAS/K-Ras 2 monoclonal antibody at 1μg/ml on various samples. Lane 1: recombinant human KRAS/K-Ras 2 (Catalog No: HF904011), 50 ng Lane 2: recombinant human KRAS/K-Ras 2 (Catalog No: HF904011), 30 ng