Anti-Human MMP13 Recombinant Antibody (40E09#)

Catalog No.

HW650013

Brand

abinScience

Species reactivity

Human

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Purity

>95% as determined by SDS-PAGE.

Isotype

VHH-mFc (IgG2a)

Applications

ELISA, FCM

Target

MMP-13, MMP13, Matrix metalloproteinase-13, Collagenase 3

Purification

Protein A/G purified from cell culture supernatant.

Endotoxin level

Please contact with the lab for this information.

Accession

P45452

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

40E09#

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Note

For research use only.

RRID

Anti-Human MMP13 Antibody (40E09#) (abinScience Cat# HW650013, RRID:AB_3729066)
_x005F_x005F_x000D_

Isotype Control

Mouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023)

Bioactivity	Detects Human MMP13 in indirect ELISAs.
Flow Cytometry	Flow-cytometry using anti-human MMP13 antibody.MDA-MB-231 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human MMP13 antibody monoclonal antibody (Catalog # HW650013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry	Flow-cytometry using anti-human MMP13 antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human MMP13 antibody monoclonal antibody (Catalog # HW650013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE	SDS-PAGE for Anti-Human MMP13 Antibody (40E09#).