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InVivoMAb Anti-Human ApoE Antibody (HJ15.4)
Overview
Catalog No. HF739040
Brand abinScience
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS pH 7.4
Purity >95% as determined by SDS-PAGE.
Isotype IgG2a, kappa
Applications ELISA, FuncS
Target Apo-E, Apolipoprotein E, APOE
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level Please contact with the lab for this information.
Accession P02649
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Clone IDHJ15.4
Note For research use only. Not suitable for clinical or therapeutic use.
Isotype Control Mouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023)
Images


Western blot
Western blot analysis was performed using anti-APOE4 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human APOE4 (Catalog No: HF739022)
Lane 2: negative control

Flow cytometry
Flow-cytometry using anti-human APOE antibody. HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human APOE monoclonal antibody (Catalog HF739040, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE
SDS-PAGE for InVivoMAb Anti-Human APOE2/3/4 Antibody (HJ15.4)