Anti-Human DPP4/CD26 Reference Antibody (YS110, RUO)

Catalog No.	HB015026
Brand	abinScience
Host species	Human
Species reactivity	Human
Form	Liquid
Storage buffer	0.01M PBS pH 7.4
Purity	>95% purity as determined by SDS-PAGE.
Clonality	Monoclonal
Isotype	IgG
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Target	TP103, Dipeptidyl peptidase IV, ADABP, Dipeptidyl peptidase 4, CD26, DPP4, Dipeptidyl peptidase IV soluble form, Dipeptidyl peptidase IV membrane form, DPP IV, ADCP2, Adenosine deaminase complexing protein 2, ADCP-2, T-cell activation antigen CD26
References	1. Hayashi M, et al. A humanized anti-CD26 monoclonal antibody inhibits cell growth of malignant mesothelioma via retarded G2/M cell cycle transition. Cancer Cell Int. 2016 Apr 30;16:35. [HB015026] 2. Yamada K, et al. Nuclear localization of CD26 induced by a humanized monoclonal antibody inhibits tumor cell growth by modulating of POLR2A transcription. PLoS One. 2013 Apr 29;8(4):e62304. [HB015026] 3. Ohnuma K, et al. Inhibition of Middle East respiratory syndrome coronavirus infection by anti-CD26 monoclonal antibody. J Virol. 2013 Dec;87(24):13892-9. [HB015026]
Purification	Protein A/G purified from cell culture supernatant.
Biological activity	YS110 (0-250 ug/mL, 48 h) inhibits cell proliferation in a concentration dependent manner in NCI-H2452 cells [1]. YS110 (2 ug/mL, 24 h) leads to a significant increase in the proportion of G2/M phase and affects the expression of related proteins in NCI-H2452 cells [1]. YS110 (2 ug/mL, 6-24 h) elevates activating phosphorylation of p38 MAPK and ERK1/2 in NCI-H2452 cells [1]. YS110 (0-20 ug/mL, 48 h) inhibits cell growth of JMN cells in a dose-dependent manner [2]. YS110 (0-4 h) relies on the caveolin pathway to induce CD26 nuclear translocation in JMN cells [2]. YS110 (2 ug/mL, 3 h) significantly inhibits the mRNA and protein levels of POLR2A in JMN cells [2]. YS110 (10 ug/mL, 30 min) effectively inhibits MERS CoV S1-Fc specific binding to CD26 in JKT-hCD26WT cells [3]. YS110 (40 ug/mL, 30 min) significantly reduces MERS CoV infection rate in Huh-7 cells [3]. Cell Proliferation Assay [1] Cell Line: NCI-H2452 cells Concentration: 0, 0.1, 2, 10 and 250 ug/mL Incubation Time: 48 h Result: Inhibited the cell proliferation of NCI-H2452 cells. Cell Cycle Analysis [1] Cell Line: NCI-H2452 cells Concentration: 2 ug/mL Incubation Time: 24 h Result: Significantly increased the proportion of G2/M phase cells with an average increase of 8 %. Western Blot Analysis [1] Cell Line: NCI-H2452 cells Concentration: 2 ug/mL Incubation Time: 6 and 24 h Result: Significantly increased the level of p21, p-cdc2, p-cdc25C, p-p38 MAPK and p-ERK1/2. RT-PCR [2] Cell Line: JMN cells Concentration: 2 ug/mL Incubation Time: 3 h Result: Significantly reduced the mRNA level of POLR2A. Western Blot Analysis [2] Cell Line: JMN cells Concentration: 2 ug/mL Incubation Time: 3 h Result: Significantly reduced the protein level of POLR2A. YS110 (5 mg/kg or 5 ug, intratumoral injection or i.p., three times per week) can significantly inhibit tumor growth in NOG mice bearing JMN xenograft [1]. YS110 (8 mg/kg or 10 ug, intratumoral injection or i.p.
Endotoxin level	< 10 EU/mg
Expression system	Mammalian cells
Accession	P27487
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative name	YS110
Clone ID	YS110
Note	For research use only. Not suitable for clinical or therapeutic use.