Anti-Human CD3 epsilon Reference Antibody (IOR-T3, RUO)

Catalog No.	HY057096
Brand	abinScience
Host species	Human
Species reactivity	Human
Form	Liquid
Storage buffer	0.01M PBS pH 7.4.
Purity	>95% as determined by SDS-PAGE.
Clonality	Monoclonal
Isotype	IgG
Applications	ELISA, Bioactivity: FCM, Functional assay, Research in vivo
Target	CD3E, CD3e, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E
References	1. Hinojosa LE, et al. Construction of a recombinant non-mitogenic anti-human CD3 antibody. Hybridoma (Larchmt). 2010;29(2):115-124. [HY057096] 2. Suarez Pestana E, et al. Growth inhibition of human lung adenocarcinoma cells by antibodies against epidermal growth factor receptor and by ganglioside GM3: involvement of receptor-directed protein tyrosine phosphatase(s). Br J Cancer. 1997;75(2):213-220. [HY057096]
Purification	Protein A/G purified from cell culture supernatant.
Biological activity	IOR-T3 (1 h) potently binds to CD3 on human PBMCs, with equivalent antigen-binding capacity to its chimeric variants T3q and T3q(Ala=Ala) [1]. IOR-T3 (70-100 ug/mL; 7 days) does not inhibit the growth of human non-small-cell lung carcinoma H125 cells when used at 100 ug/mL for 7 days [2]. Cell Proliferation Assay [2] Cell Line: human non-small-cell lung carcinoma H125 cells Concentration: 70, 90, 100 ug/mL Incubation Time: 7 days Result: Showed no inhibitory effect on H125 cell growth, with cell protein content remaining at 100% of control levels.
Endotoxin level	< 10 EU/mg as determined by LAL method.
Accession	P07766
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative name	IOR-T3, IORT3, IORT3
Clone ID	IOR-T3
Note	For research use only. Not suitable for clinical or therapeutic use.