Anti-Human CD30/TNFRSF8 Reference Antibody (HeFi-1, RUO)

Catalog No.	HB038056
Brand	abinScience
Host species	Human
Species reactivity	Human
Form	Liquid
Storage buffer	0.01M PBS pH 7.4
Purity	>95% purity as determined by SDS-PAGE.
Clonality	Monoclonal
Isotype	IgG
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Target	CD30L receptor, Ki-1 antigen, CD30, TNFRSF8, D1S166E, Tumor necrosis factor receptor superfamily member 8, Lymphocyte activation antigen CD30
References	1. Zhang M, et al. Effective therapy for a murine model of human anaplastic large-cell lymphoma with the anti-CD30 monoclonal antibody, HeFi-1, does not require activating Fc receptors. Blood. 2006 Jul 15;108(2):705-10. [HB038056] 2. Berro AI, et al. Increased expression and activation of CD30 induce apoptosis in human blood eosinophils. J Immunol. 2004 Aug 1;173(3):2174-83. [HB038056]
Purification	Protein A/G purified from cell culture supernatant.
Biological activity	HeFi-1 (0.1 ng/uL, 1 h) can bind specifically to Karpas299 cells with a binding rate of over 90 % [1]. HeFi-1 (20 ug/mL, 48 h) can significantly inhibit the proliferation of Karpas299 cells and induces G1 phase blockade [1]. HeFi-1 (20 ug/mL, 12-24 h) can significantly induce apoptosis in eosinophil [2]. Cell Proliferation Assay [1] Cell Line: Karpas299 cells Concentration: 20 ug/mL Incubation Time: 48 h Result: Significantly inhibited cell proliferation with an inhibition rate of 40%, superior to Daclizumab (). Cell Cycle Analysis [1] Cell Line: Karpas299 cells Concentration: 20 ug/mL Incubation Time: 48 h Result: Induced G1 phase arrest and a decrease in S phase cells. HeFi-1 (100 ug, i.v., once weekly, for 4 weeks) can effectively prolong survival in SCID/NOD wild-type and FcRγ -/- mice bearing Karpas299 lymphoma [1].
Endotoxin level	< 10 EU/mg
Expression system	Mammalian cells
Accession	P28908
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative name	HeFi-1, HeFi1, HeFi1
Clone ID	HeFi-1
Note	For research use only. Not suitable for clinical or therapeutic use.