Rat Anti-Human Glypican 3 Recombinant Antibody (YP7)

Catalog No.

HW310053

Brand

abinScience

Tested applications

WB, FCM

Species reactivity

Human

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Purity

>95% as determined by SDS-PAGE.

Isotype

IgG1, kappa

Applications

ELISA, FCM, WB

Target

GPC3, MXR7, GTR2-2, Glypican-3, Intestinal protein OCI-5, OCI5

Purification

Protein A/G purified from cell culture supernatant.

Endotoxin level

Please contact with the lab for this information.

Accession

P51654

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

YP7

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Note

For research use only.

RRID

Rat Anti-Human GPC3 Antibody (YP7) (abinScience Cat# HW310053, RRID:AB_3729294)

Isotype Control

Rat IgG1, kappa Recombinant Isotype Control Antibody (RTK2071) (RV080407)

SDS-PAGE	SDS-PAGE for Rat Anti-Human GPC3 Antibody (YP7).
Flow CytoMetry	Flow-cytometry using anti-human GPC3 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human GPC3 monoclonal antibody (Catalog: HW310053) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Rat IgG H&L Polyclonal Antibody, FITC (abinScience: ？) and cells analysed on a NovoCyte Flow Cytometer.
Bioactivity	Detects recombinant human Glypican-3/GPC3 (Cat No.: HW310011) in indirect ELISA.
Bioactivity	Detects recombinant mouse Glypican-3/GPC3 (Cat No.: MW310011) in indirect ELISA.
Western blot	Western blot analysis was performed using anti-GPC3 monoclonal antibody at 1ug/mL on various samples. Lane 1: recombinant human GPC3 (Catalog No: HW310011) Lane 2: negative control