| Catalog No. | HV181010 | ||||||
| Brand | abinScience | ||||||
| Species reactivity | Human | ||||||
| Form | Liquid | ||||||
| Storage buffer | 0.01M PBS pH 7.4 | ||||||
| Purity | >95% as determined by SDS-PAGE. | ||||||
| Isotype | IgG | ||||||
| Applications | ELISA, WB, FCM, Neutralization | ||||||
| Target | Tumor susceptibility gene 101 protein, ESCRT-I complex subunit TSG101, TSG101 | ||||||
| Purification | Protein A/G purified from cell culture supernatant. | ||||||
| Endotoxin level | Please contact with the lab for this information. | ||||||
| Accession | Q99816 | ||||||
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||
| Reconstitution | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. | ||||||
| Clone ID | Iv0253 | ||||||
| Product Usage Information |
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| Note | For research use only. Not suitable for clinical or therapeutic use. | ||||||
| Isotype Control | Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507) |
InVivoMAb Anti-Human TSG101 Antibody (Iv0253)
Overview
Images
 Western blot |
Western blot analysis was performed using anti-TSG101 monoclonal antibody at 1ug/mL on various samples. Lane 1: SH-SY5Y cell lysate Lane 2: K562 cell lysate Lane 3: NIH3T3 cell lysate Lane 4: PC-12 cell lysate Lane 5: Lncap cell lysate Lane 6: 293T cell lysate Lane 7: Mouse brain lysate Lane 8: Rat brain lysate Lane 9: Rat heart lysate |
 Flow cytometry |
Flow-cytometry using APC anti-human TSG101 antibody. Jurkat cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an APC anti-human TSG101 monoclonal antibody (Catalog HV181010, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer. |
 Flow cytometry |
Flow-cytometry using PE anti-human TSG101 antibody. Jurkat cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human TSG101 monoclonal antibody (Catalog HV181010, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer. |