| Catalog No. | HV233013 | ||||
| Brand | abinScience | ||||
| Species reactivity | Human | ||||
| Form | Liquid | ||||
| Storage buffer | 0.01M PBS pH 7.4 | ||||
| Purity | >95% as determined by SDS-PAGE. | ||||
| Isotype | IgG2a | ||||
| Applications | ELISA, FCM, IF, IP | ||||
| Target | Dnmt3a, DNA MTase HsaIIIA, DNMT3A, M.HsaIIIA, DNA (cytosine-5)-methyltransferase 3A, DNA methyltransferase HsaIIIA | ||||
| Purification | Protein A/G purified from cell culture supernatant. | ||||
| Endotoxin level | Please contact with the lab for this information. | ||||
| Accession | Q9Y6K1 | ||||
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | ||||
| Clone ID | SAA0199 | ||||
| Product Usage Information |
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| Note | For research use only. | ||||
| RRID | Anti-Human DNMT3A IP Antibody (SAA0199) (abinScience Cat# HV233013, RRID:AB_3729733) |
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| Isotype Control | Mouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023) |
Anti-Human DNMT3A IP Recombinant Antibody (SAA0199)
Overview
Images
 Flow Cytometry |
Flow-cytometry using anti-human DNMT3A antibody.293T cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human DNMT3A antibody monoclonal antibody (Catalog # HV233013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 Flow Cytometry |
Flow-cytometry using anti-human DNMT3A antibody.HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human DNMT3A antibody monoclonal antibody (Catalog # HV233013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 Flow Cytometry |
Flow-cytometry using anti-human DNMT3A antibody.Human Jurkat cell line were stained with an irrelevant antibody (Blue Histogram) or an anti-human DNMT3A antibody monoclonal antibody (Catalog # HV233013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 Flow Cytometry |
Flow-cytometry using anti-human DNMT3A antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human DNMT3A antibody monoclonal antibody (Catalog # HV233013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 SDS-PAGE |
SDS-PAGE for Anti-Human DNMT3A IP Antibody (SAA0199). |
 Flow CytoMetry |
Flow-cytometry using FITC anti-human DNMT3A antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human DNMT3A monoclonal antibody (Catalog: HV233013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer. |
 Flow CytoMetry |
Flow-cytometry using PE anti-human DNMT3A antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human DNMT3A monoclonal antibody (Catalog: HV233013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer. |