| Catalog No. | HV451016 |
| Brand | abinScience |
| Species reactivity | Human |
| Form | Liquid |
| Storage buffer | 0.01M PBS pH 7.4 |
| Purity | >95% purity as determined by SDS-PAGE. |
| Isotype | IgG1-kappa |
| Applications | ELISA, Bioactivity: FACS, Functional assay, Research in vivo |
| Target | CD318, Transmembrane and associated with src kinases, TRASK, Subtractive immunization M plus HEp3-associated 135 kDa protein, SIMA135, Membrane glycoprotein gp140, CUB domain-containing protein 1, CDCP1 |
| References | 1. Gwendlyn Kollmorgen, et al. Antibody mediated CDCP1 degradation as mode of action for cancer targeted therapy. Mol Oncol. 2013 Dec;7(6):1142-51. [HV451016] |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin level | < 10 EU/mg |
| Expression system | Mammalian cells |
| Accession | Q9H5V8 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Alternative name | RG7287 |
| Clone ID | RG7287 |
| Note | For research use only. Not suitable for clinical or therapeutic use. |
| Isotype Control | Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507) |
Anti-Human CDCP1 Reference Antibody (RG7287, RUO)
Overview
Images
 Bioactivity |
Detects CD318 in indirect ELISA. |
 SDS-PAGE |
SDS-PAGE for Research Grade Anti-Human CD318/CDCP1 (RG7287). |
 Flow CytoMetry |
Flow-cytometry using APC anti-human CD318 antibody. HT-29 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD318 monoclonal antibody (Catalog: HV451016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer. |
 Flow CytoMetry |
Flow-cytometry using PE anti-human CD318 antibody. HT-29 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD318 monoclonal antibody (Catalog: HV451016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer. |
 Flow cytometry |
Flow-cytometry using anti-human CD318 antibody. HT29 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD318 monoclonal antibody (Catalog HV451016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer. |