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Anti-Human CEACAM-5/CD66e Reference Antibody (SGM 101, RUO)
Overview
Catalog No. HY546106
Brand abinScience
Tested applications WB, FCM
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS pH 7.4
Purity >95% purity as determined by SDS-PAGE.
Isotype IgG1-kappa
Applications ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Target Meconium antigen 100, CEACAM5, CEA, CD66e, Carcinoembryonic antigen-related cell adhesion molecule 5, Carcinoembryonic antigen
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level < 10 EU/mg
Expression system Mammalian cells
Accession P06731
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative nameSGM-101, SGM101, SGM101
Clone IDSGM 101
Note For research use only. Not suitable for clinical or therapeutic use.
Isotype Control Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)
Images


SDS-PAGE
SDS-PAGE for Research Grade Anti-Human CD66e/CEA/CEACAM5 (SGM 101).

Bioactivity
Detects recombinant human CD66e/CEA/CEACAM5 (Cat No.: HY546011) in indirect ELISA.

Western blot
Western blot analysis was performed using anti-CD66e/CEA/CEACAM5 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CD66e/CEA/CEACAM5 (Catalog No: HY546011)
Lane 2: negative control

Flow cytometry
Flow-cytometry using PE anti-human CD66e antibody. HPAC cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD66e monoclonal antibody (Catalog HY546106, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

Flow cytometry
Flow-cytometry using FITC anti-human CD66e antibody. HPAC cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD66e monoclonal antibody (Catalog HY546106, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.