下载
Anti-Human CEACAM-5/CD66e Reference Antibody (SGM 101, RUO)
Overview
Catalog No. HY546106
Brand abinScience
Host species Human
Tested applications WB, FCM
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS pH 7.4.
Purity >95% as determined by SDS-PAGE.
Clonality Monoclonal
Isotype IgG1-kappa
Applications ELISA, Bioactivity: FCM, Functional assay, Research in vivo
Target CD66e, CEA, CEA cell adhesion molecule 5, CEACAM5, Carcinoembryonic antigen, Carcinoembryonic antigen-related cell adhesion molecule 5, Cell adhesion molecule CEACAM5, Meconium antigen 100
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level < 10 EU/mg as determined by LAL method.
Accession P06731
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative nameSGM-101, SGM101, SGM101
Clone IDSGM 101
Note For research use only. Not suitable for clinical or therapeutic use.
Isotype Control Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)
Images


SDS-PAGE		 SDS-PAGE for Research Grade Anti-Human CD66e/CEA/CEACAM5 (SGM 101).

Bioactivity		 Detects recombinant human CD66e/CEA/CEACAM5 (Cat No.: HY546011) in indirect ELISA.

Western blot		 Western blot analysis was performed using anti-CD66e/CEA/CEACAM5 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CD66e/CEA/CEACAM5 (Catalog No: HY546011)
Lane 2: negative control

Flow cytometry		 Flow-cytometry using PE anti-human CD66e antibody. HPAC cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD66e monoclonal antibody (Catalog HY546106, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

Flow cytometry		 Flow-cytometry using FITC anti-human CD66e antibody. HPAC cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD66e monoclonal antibody (Catalog HY546106, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.