| Catalog No. | HY196066 |
| Brand | abinScience |
| Host species | Human |
| Species reactivity | Human |
| Form | Liquid |
| Storage buffer | 0.01M PBS pH 7.4 |
| Purity | >95% purity as determined by SDS-PAGE. |
| Clonality | Monoclonal |
| Isotype | IgG |
| Applications | ELISA, Bioactivity: FACS, Functional assay, Research in vivo |
| Target | Tyrosine-protein kinase Met, Hepatocyte growth factor receptor, Proto-oncogene c-Met, Scatter factor receptor, HGF receptor, SF receptor, HGF/SF receptor, MET |
| References | 1. Hultberg A, et al. Depleting MET-Expressing Tumor Cells by ADCC Provides a Therapeutic Advantage over Inhibiting HGF/MET Signaling. Cancer Res. 2015 Aug 15;75(16):3373-83. [HY196066] 2. V Morello, et al. Abstract P2-05-17: ARGX-111 depletes MET-expressing circulating tumor cells via enhanced ADCC, resulting in inhibition of metastasis. Cancer Res 15 February 2016; 76 (4_Supplement): P2–05–17. [HY196066] |
| Purification | Protein A/G purified from cell culture supernatant. |
| Biological activity | ARGX-111 binds to native MET with an EC 50 ranging from 0.1 nmol/L to 0.7 nmol/L, competes with HGF displaying an IC 50 of 1.3 nmol/L and an IMAX of 95% [1]. ARGX-111 inhibits HGF-induced MET autophosphorylation in A549 cells (IC 50 = 5.3 nmol/L, IMAX = 74%), inhibits MET autophosphorylation in both MKN-45 cells (IC 50 = 0.2 nmol/L, IMAX = 63%) and EBC-1 cells (IC 50 = 12.7 nmol/L, IMAX = 69%) [1]. ARGX-111 neutralizes the promigratory effect of HGF, inhibits HGF-dependent colony formation by 78% in A549 cells [1]. ARGX-111 (1-10 nM/L) inhibits HGF-induced branched tubule formation in SV40 T-antigen-transformed LOC human kidney epithelial cell spheroids [1]. ARGX-111 (0.1-100 h) promotes a rapid decrease in MET expression in MKN-45, EBC-1 and MDA-MB 231 cells [1]. ARGX-111 (24-48 h) does not promote receptor shedding but reduced total MET protein levels in MKN-45 cells [1]. ARGX-111 (0-1000 nM/L) induces dose-dependent lysis in MKN-45, NCI-H441, A549 cells [1]. ARGX-111 induces antibody-dependent cellular phagocytosis (ADCP) in MKN-45 and 786-O cells [1]. ARGX-111 (0-667 nM/L) promotes dose-dependent ADCC in ALDH-1-sorted MDA-MB-231, CRCM168, CRCM174, CRCM389 cells [1]. |
| Endotoxin level | < 10 EU/mg |
| Expression system | Mammalian cells |
| Accession | P08581 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Alternative name | ARGX-111, ARGX111, ARGX111 |
| Clone ID | ARGX-111 |
| Note | For research use only. Not suitable for clinical or therapeutic use. |
Anti-Human HGFR/c-MET Reference Antibody (ARGX-111, RUO)
Overview
Images
 SDS-PAGE |
SDS-PAGE for Research Grade Anti-Human MET/c-Met/HGFR (ARGX-111) |
 Bioactivity |
Detects recombinant human MET/c-Met/HGFR (Catalog No: HY196021) in indirect ELISA |