Research Grade Tenecteplase

Catalog No.

HF934016

Brand

abinScience

Species reactivity

Human

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Purity

>95% purity as determined by SDS-PAGE.

Clonality

Monoclonal

Applications

ELISA, Functional assay, Research in vivo

Target

t-plasminogen activator, tPA, t-PA, Reteplase, Tissue-type plasminogen activator, Alteplase, PLAT

References

1. J Bastecký, et al. [Actilyse (a recombinant tissue-type plasminogen activator] in the treatment of acute myocardial infarct. Comparison of the effects of Actilyse and streptokinase]. Vnitr Lek. 1994 May;40(5):293-8. [HF934016]
2. Micieli G, et al. Safety and efficacy of alteplase in the treatment of acute ischemic stroke. Vasc Health Risk Manag. 2009;5(1):397-409. [HF934016]
3. Orset C, et al. Efficacy of Alteplase in a Mouse Model of Acute Ischemic Stroke: A Retrospective Pooled Analysis. Stroke. 2016 May;47(5):1312-1318. [HF934016]
4. Kimura S, et al. Cytotoxic effects of alteplase, a recombinant tissue plasminogen activator, on human retinal pigment epithelial cells. Jpn J Ophthalmol. 2021 Sep;65(5):731-739. View More [HF934016]

Purification

Purified by ion exchange chromatography.

Biological activity

Alteplase (8.3 and 250 ug/mL, 12 h) shows a signifcant reduction in viability of human fetal retinal pigment epithelial (hfRPE cells) [4]. Alteplase (8.3 and 250 ug/mL, 0-6 h) signifcantly decreases the transepithelial resistance in a time-dependent manner and induces cell death in hfRPE cells [4]. Alteplase (0.9-10 mg/kg, i.v.

Endotoxin level

< 10 EU/mg

Expression system

Mammalian cells

Accession

P00750

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternative name

Tnkase, Dp00031, TNK-tPA, Metalyse, 191588-94-0

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Note

For research use only. Not suitable for clinical or therapeutic use.