| Catalog No. | HT018016 |
| Brand | abinScience |
| Species reactivity | Human |
| Form | Liquid |
| Storage buffer | 0.01M PBS pH 7.4 |
| Purity | >95% purity as determined by SDS-PAGE. |
| Isotype | IgG |
| Applications | ELISA, Bioactivity: FACS, Functional assay, Research in vivo |
| Target | NPPase, PD-Ibeta, Phosphodiesterase I beta, PDNP3, CD203c, Ectonucleotide pyrophosphatase/phosphodiesterase family member 3, E-NPP 3, ENPP3, NPP3, Phosphodiesterase I/nucleotide pyrophosphatase 3, Nucleotide diphosphatase |
| References | 1. Kollmannsberger C, et al. A Randomized Phase II Study of AGS-16C3F Versus Axitinib in Previously Treated Patients with Metastatic Renal Cell Carcinoma. Oncologist. 2021 Mar;26(3):182-e361. [HT018016] 2. Zhang Y, et al. In vitro and in vivo efficacy of an anti-CD203c conjugated antibody (AGS-16C3F) in mouse models of advanced systemic mastocytosis. Blood Adv. 2019 Feb 26;3(4):633-643. [HT018016] |
| Purification | Protein A/G purified from cell culture supernatant. |
| Biological activity | AGS-16C3F (0.001-1350 nM, 96 h) has IC 50 of 1.1 nM, 2.73 nM, 109.9 nM and 146.5 nM in ROSA KIT D816V, ROSA KIT D816V-Gluc, HMC-1.1 and HMC-1.2 cells, respectively, shows potent inhibitory activity against the proliferation of ROSA cells with high expression of CD203c [2]. AGS-16C3F (0.5-100 nM, 96-120 h) induces apoptosis and kills tumor cells with high expression of CD203c in ROSA KIT D816V, ROSA KIT D816V-Gluc, HMC-1.1 and HMC-1.2 cells [2]. Apoptosis Analysis [2] Cell Line: ROSA KIT D816V, ROSA KIT D816V-Gluc, HMC-1.1 and HMC-1.2 cells Concentration: 0.5-100 nM Incubation Time: 96-120 h Result: Significantly increased the proportion of apoptotic cells in ROSA cells (dose-dependent). Had a low apoptosis rate in HMC-1 cells. AGS-16C3F (1,4 mg/kg, i.v. once every 4 days, 4 times in total) significantly reduces tumor burden and prolongs survival in the NOD-SCID γ mice model transplants with ROSA KIT D816V-Gluc cells without significant toxicity [2]. |
| Endotoxin level | < 10 EU/mg |
| Expression system | Mammalian cells |
| Accession | O14638 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Alternative name | AGS-16C3F, AGS16C3F, AGS16C3F |
| Clone ID | AGS-16C3F |
| Note | For research use only. Not suitable for clinical or therapeutic use. |
| Isotype Control | Human IgG2 Recombinant Isotype Control Antibody (HyHEL-10) (HV988107) |
Anti-Human ENPP‑3/CD203c Reference Antibody (AGS-16C3F, RUO)
Overview
Images
 Flow cytometry |
HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-CD203c monoclonal antibody (Catalog HT018016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analyzed on a NovoCyte Flow Cytometer. |
 Flow cytometry |
Untransfected cells or cells transfected with CD203c/ENPP3 were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD203c monoclonal antibody (Catalog HT018016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analyzed on a NovoCyte Flow Cytometer. |