Anti-Human Flt-3/Flk-2 Reference Antibody (IMC-EB10, RUO)

Catalog No.	HW794036
Brand	abinScience
Host species	Human
Species reactivity	Human
Form	Liquid
Storage buffer	0.01M PBS pH 7.4
Purity	>95% purity as determined by SDS-PAGE.
Clonality	Monoclonal
Isotype	IgG
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Target	FLT3, Stem cell tyrosine kinase 1, Fetal liver kinase-2, STK-1, STK1, FLK2, CD135, Receptor-type tyrosine-protein kinase FLT3, Fms-like tyrosine kinase 3, FL cytokine receptor, FLK-2, FLT-3
References	1. Piloto O, et al. IMC-EB10, an anti-FLT3 monoclonal antibody, prolongs survival and reduces nonobese diabetic/severe combined immunodeficient engraftment of some acute lymphoblastic leukemia cell lines and primary leukemic samples. Cancer Res. 2006 May 1;66(9):4843-51 [HW794036] 2. Erik Corcoran, et al. Abstract #2923: Preclinical pharmacokinetic and pharmacodynamic evaluation of the anti-FLT3 monoclonal antibody IMC-EB10. Cancer Res 1 May 2009; 69 (9_Supplement): 2923. [HW794036] 3. David Surguladze, et al. Synergistic Anti-Leukemic Effect of Combination Therapy with Anti-FLT3 Antibody.IMC-EB10 and Methotrexate,Blood,Volume 112, Issue 11,2008,Page 3964,ISSN 0006-4971, [HW794036] 4. Li Y, et al. Suppression of leukemia expressing wild-type or ITD-mutant FLT3 receptor by a fully human anti-FLT3 neutralizing antibody. Blood.2004 Aug 15;104(4):1137-44. View More [HW794036]
Purification	Protein A/G purified from cell culture supernatant.
Biological activity	IMC-EB10 (10 ug/mL, 1 h) reduces FLT3 and phosphorylation of downstream signaling proteins STAT5, Akt, and MAPK in Hb1119 and SEM-K2 cells, but activates them in REH and RS411 cells [1]. IMC-EB10 (10 ug/mL, 1 h) activates FLT3 and downstream Akt in ALLs [1]. IMC-EB10 (0.4-200 nM, 1 h) inhibits FLT3-Fc-induced phosphorylation of wild-type FLT3 and ligand-independent constitutive phosphorylation of ITD-mutant FLT3 in EOL-1, EM3, BaF3-ITD and MV4;11 cells [4]. IMC-EB10 (0.4-200 nM, 1 h) inhibits FLT3-Fc-induced phosphorylation of MAPK, AKT and Stat5 in EOL-1, EM3, BaF3-ITD and MV4;11 cells [4]. IMC-EB10 (1.5-100 nM, 1 h) inhibits proliferation of FLT3-Fc-induced proliferation of EOL-1 and BaF3-ITD cells [4]. Western Blot Analysis [1] Cell Line: Hb1119, SEM-K2 cells Concentration: 10 ug/mL Incubation Time: 1 h Result: Reduced FLT3 and phosphorylation of downstream signaling proteins STAT5, Akt, and MAPK. Western Blot Analysis [1] Cell Line: REH, RS411 cells Concentration: 10 ug/mL Incubation Time: 1 h Result: Activated FLT3 and phosphorylation of downstream signaling proteins STAT5, Akt, and MAPK. Western Blot Analysis [1] Cell Line: ALLs Concentration: 10 ug/mL Incubation Time: 1 h Result: Activated FLT3 and downstream Akt. . Blocked the phosphorylation and constitutive phosphorylation of MAPK, inhibited FL-induced phosphorylation of AKT and Stat5 phosphorylation in EOL-1 and BaF3-ITD cells. Parmacokinetics Species Dose Route T 1/2 AUC 0-inf Mice [2] 20 mg/kg i.p. 96 h 38327 ug·h/mL IMC-EB10 (400 ug/10 mg/kg, i.p.
Endotoxin level	< 10 EU/mg
Expression system	Mammalian cells
Accession	P36888
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative name	IMC-EB10
Clone ID	IMC-EB10
Note	For research use only. Not suitable for clinical or therapeutic use.