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Anti-Human CD127/IL-7R alpha Reference Antibody (Bempikibart, RUO)
Overview
Catalog No. HB016026
Brand abinScience
Tested applications FCM
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS pH 7.4
Purity >95% purity as determined by SDS-PAGE.
Isotype IgG1-kappa
Applications ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Target CD127, CDw127, IL-7 receptor subunit alpha, IL-7R subunit alpha, IL-7R-alpha, IL-7RA, IL7R, Interleukin-7 receptor subunit alpha
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level < 10 EU/mg
Expression system Mammalian cells
Accession P16871
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative nameCAS: 2622254-57-1
Note For research use only. Not suitable for clinical or therapeutic use.
Isotype Control Human IgG1 (LALAPG) Recombinant Isotype Control Antibody (HyHEL-10) (HP688307)
Human IgG1 (LALA) Recombinant Isotype Control Antibody (HyHEL-10) (HP688107)
Images


SDS-PAGE
SDS-PAGE for Research Grade Bempikibart.

Flow cytometry
Flow-cytometry using APC anti-human CD127 antibody. 293T transfected cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD127 monoclonal antibody (Catalog HB016026, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

Flow cytometry
Flow-cytometry using PE anti-human CD127 antibody. 293T transfected cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD127 monoclonal antibody (Catalog HB016026, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.