| Catalog No. | YP144013 | ||||
| Brand | abinScience | ||||
| Host species | Mouse | ||||
| Species reactivity | General | ||||
| Form | Liquid | ||||
| Storage buffer | 0.01M PBS pH 7.4. | ||||
| Purity | >95% as determined by SDS-PAGE. | ||||
| Clonality | Monoclonal | ||||
| Isotype | IgG1, kappa | ||||
| Applications | IF, IHC, IP, WB | ||||
| Target | GFP Tag, GFP-Tag, Green fluorescent protein | ||||
| Purification | Protein A/G purified from cell culture supernatant. | ||||
| Endotoxin level | < 10 EU/mg | ||||
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | ||||
| Clone ID | N86/38.1 | ||||
| Product Usage Information |
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| Note | For research use only. |
Anti-GFP Recombinant Antibody (N86/38.1)
Overview
Images
 SDS-PAGE |
SDS-PAGE for Anti-GFP Tag Antibody (N86/38.1) |
 Western blot |
Western blot analysis was performed using anti-GFP tag monoclonal antibody at 1ug/mL on various samples. Lane 1: Recombinant EGFP |
 Immunofluorescence |
Immunofluorescent analysis of 4% PFA-fixed and 0.1% Triton X-100-permeabilized 293T cells transfected with pEGFP-N1 using GFP antibody (Cat. No. ZP140013) at 20 μg/mL. CY3-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) was used as the secondary antibody, and nuclei were counterstained with DAPI (blue). |