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Anti-GFP Tag Recombinant Antibody (N86/38.1)
Overview
Catalog No. YP144013
Brand abinScience
Species reactivity General
Form Liquid
Storage buffer 0.01M PBS pH 7.4
Isotype IgG1, kappa
Applications IF, IHC, IP, WB
Target GFP Tag, GFP-Tag, Green fluorescent protein
Purification Protein A/G purified from cell culture supernatant.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone IDN86/38.1
Product Usage Information
ApplicationDilution
WB 1:500-1:2000
Note For research use only.
Images


SDS-PAGE
SDS-PAGE for Anti-GFP Tag Antibody (N86/38.1)

Western blot
Western blot analysis was performed using anti-GFP tag monoclonal antibody at 1ug/mL on various samples.
Lane 1: Recombinant EGFP

Immunofluorescence
Immunofluorescent analysis of 4% PFA-fixed and 0.1% Triton X-100-permeabilized 293T cells transfected with pEGFP-N1 using GFP antibody (Cat. No. ZP140013) at 20 μg/mL. CY3-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) was used as the secondary antibody, and nuclei were counterstained with DAPI (blue).