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Human MDA5 Recombinant Protein
Overview
Catalog No. XV365031
Brand abinScience
Form Liquid
Storage buffer 20 mM Tris-HCl, pH 8.0, 100mM NaCl, 1mM DTT, 10% Glycerol.
Purity >95% as determined by SDS-PAGE.
Applications As a diagnostic tool for Clinically Amyopathic Dermatomyositis (CADM).
Endotoxin level Please contact with the lab for this information.
Expression system Confidential
Accession Q9BYX4
Protein length Confidential
Nature Recombinant
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Reconstitution Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.

RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Alternative nameMDA-5, IFIH1, Helicard, Clinically amyopathic dermatomyositis autoantigen 140 kDa, Interferon-induced with helicase C domain protein 1, Helicase with 2 CARD domains, RNA helicase-DEAD box protein 116, RIG-I-like receptor 2, Interferon-induced helicase C domain-containing protein 1, MDA5, Murabutide down-regulated protein, Melanoma differentiation-associated protein 5, CADM-140 autoantigen, RH116, RLR-2
Species Homo sapiens (Human)
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Predicted molecular weight 118.04 kDa
Note For research use only.
Images


SDS-PAGE
SDS-PAGE for Recombinant Human CADM-140/MDA5 Protein

Bioactivity
Detected by SAA2091 (Catalog No: HV365013) in indirect ELISA.