Anti-Human PSCA Reference Antibody (Mk-4721, RUO)

Catalog No.	HT361026
Brand	abinScience
Species reactivity	Human
Form	Liquid
Storage buffer	0.01M PBS pH 7.4
Purity	>95% purity as determined by SDS-PAGE.
Isotype	scFv-hFc
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Target	Prostate stem cell antigen, PSCA
References	1. Tony Navas, et al. Abstract 4566: AGS-1C4D4: A fully human anti-PSCA antibody inhibits tumor formation and metastasis in orthotopic models of pancreatic cancer. Cancer Res (2011) 71 (8_Supplement): 4566. [HT361026]
Purification	Protein A/G purified from cell culture supernatant.
Biological activity	AGS-1C4D4 displays potent ADCC activity against PSCA-expressing HPAC and Panc0203 pancreatic cell lines, with activity greatly reduced by deglycosylation, and is more potent than the hybridoma-derived AGS-PSCA antibody in this assay [1]. AGS-1C4D4 exhibits CDC activity against PSCA-expressing recombinant B300.19 cells, with activity greatly reduced by deglycosylation, and is equally effective as the hybridoma-derived AGS-PSCA antibody in this assay [1]. AGS-1C4D4 binds to PSCA with a K d of 0.2 nM, displaying similar binding affinity to the hybridoma-derived AGS-PSCA antibody [1]. AGS-1C4D4 significantly inhibits established pancreatic tumor growth and metastasis in orthotopic HPAC xenograft models when combined with Gemcitabine (), and also significantly inhibits pancreatic tumor formation, local invasion, and distant metastasis in the same orthotopic xenograft model [1].
Endotoxin level	< 10 EU/mg
Expression system	Mammalian cells
Accession	O43653
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative name	ProscamabA11, MK-4721
Note	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Human IgG1 Recombinant Isotype Control Antibody (HyHEL-10) (HV080407) Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)