| Catalog No. | HY286566 |
| Brand | abinScience |
| Host species | Human |
| Tested applications | WB, FCM |
| Species reactivity | Human |
| Form | Liquid |
| Storage buffer | 0.01M PBS pH 7.4. |
| Purity | >95% as determined by SDS-PAGE. |
| Clonality | Monoclonal |
| Isotype | scFv-Human IgG1, kappa |
| Applications | ELISA, Bioactivity: FCM, Functional assay, Research in vivo |
| Target | CD340, EC:2.7.10.1, ERBB2, HER2, MLN 19, MLN19, Metastatic lymph node gene 19 protein, NEU, NGL, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, Receptor tyrosine-protein kinase erbB-2, Tyrosine kinase-type cell surface receptor HER2, p185erbB2 |
| References | 1. Pegram MD, et al. First-in-Human, Phase 1 Dose-Escalation Study of Biparatopic Anti-HER2 Antibody-Drug Conjugate MEDI4276 in Patients with HER2-positive Advanced Breast or Gastric Cancer. Mol Cancer Ther. 2021 Aug;20(8):1442-1453. [HY286566] |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin level | < 10 EU/mg as determined by LAL method. |
| Accession | P04626 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Alternative name | MEDI4276 |
| Clone ID | MEDI4276 |
| Note | For research use only. Not suitable for clinical or therapeutic use. |
| Isotype Control | Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507) |
Anti-Human ErbB2/Her2 Reference Antibody (MEDI4276, RUO)
Overview
Images
 SDS-PAGE |
SDS-PAGE for Research Grade Anti-Human CD340/ERBB2/HER2/NEU (MEDI4276) |
 Flow cytometry |
Flow-cytometry using anti-human CD340 antibody. SK-BR-3 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD340 monoclonal antibody (Catalog HY286566, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 Bioactivity |
Detects recombinant CD340 (Catalog No: HY286011) in indirect ELISA. |
 Western blot |
Lane 1: recombinant human CD340/ERBB2 (Catalog No: HY286011) Lane 2: 1*loading buffer |